CD146 positive human dental pulp stem cells promote regeneration of dentin/pulp-like structures.

ABSTRACT: CD146 and STRO-1 are endothelial biomarkers that are co-expressed on the cellular membranes of blood vessels within human dental pulp tissue. This study characterized the percentage of dentin-like structures produced by CD146-positive (CD146⁺) human dental pulp stem cells (DPSCs), compared with their CD146-negative (CD146^-) counterparts. DPSC populations were enriched using magnetic-activated cell sorting (MACS), yielding CD146⁺ and CD146^- cells, as well as mixtures composed of 25% CD146⁺ cells and 75% CD146^- cells (CD146^+/-). Cell growth assays indicated that CD146⁺ cells exhibit an approximate 3-4 h difference in doubling time, compared with CD146^- cells. Cell cycle distributions were deter

SUBMITTER: Matsui M 

PROVIDER: S-EPMC5852189 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature