Cgnl1, an endothelial junction complex protein, regulates GTPase mediated angiogenesis.

ABSTRACT:

Aims

The formation of cell-cell and cell-extra cellular matrix (ECM) contacts by endothelial cells (ECs) is crucial for the stability and integrity of a vascular network. We previously identified cingulin-like 1 (Cgnl1) in a transcriptomic screen for new angiogenic modulators. Here we aim to study the function of the cell-cell junction associated protein Cgnl1 during vessel formation.

Methods and results

Unlike family member cingulin, Cgnl1 expression is enriched in ECs during vascular growth. Cgnl1 is important for the formation of multicellular tubule structures, as shown in vitro using loss-of function assays in a 3D matrix co-culture system that uses primary human ECs and supporting mural cells. Further studies revealed that Cgnl1 regulates vascular growth by promoting V

SUBMITTER: Chrifi I 

PROVIDER: S-EPMC5852532 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature