Differential eNOS-signalling by platelet subpopulations regulates adhesion and aggregation.

ABSTRACT:

Aims

In addition to maintaining haemostasis, circulating blood platelets are the cellular culprits that form occlusive thrombi in arteries and veins. Compared to blood leucocytes, which exist as functionally distinct subtypes, platelets are considered to be relatively simple cell fragments that form vascular system plugs without a differentially regulated cellular response. Hence, investigation into platelet subpopulations with distinct functional roles in haemostasis/thrombosis has been limited. In our present study, we investigated whether functionally distinct platelet subpopulations exist based on their ability to generate and respond to nitric oxide (NO), an endogenous platelet inhibitor.

Methods and results

Utilizing highly sensitive and selective flow cytometry protoc

SUBMITTER: Radziwon-Balicka A 

PROVIDER: S-EPMC5852541 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature