HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry.

ABSTRACT: The chromosomal passenger complex (CPC) is directed to centromeres during mitosis via binding to H3T3ph and Sgo1. Whether and how heterochromatin protein 1α (HP1α) influences CPC localisation and function during mitotic entry is less clear. Here, we alter HP1α dynamics by fusing it to a CENP-B DNA-binding domain. Tethered HP1 strongly recruits the CPC, destabilising kinetochore-microtubule interactions and activating the spindle assembly checkpoint. During mitotic exit, the tethered HP1 traps active CPC at centromeres. These HP1-CPC clusters remain catalytically active throughout the subsequent cell cycle. We also detect interactions between endogenous HP1 and the CPC during G₂ HP1α and HP1γ cooperate to recruit the CPC to active foci in a CDK1-independent process. Live cell tra

SUBMITTER: Ruppert JG 

PROVIDER: S-EPMC5852645 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature