Ontology highlight
ABSTRACT:
SUBMITTER: Masliah G
PROVIDER: S-EPMC5852647 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
The EMBO journal 20180215 6
The accurate cleavage of pre-micro(mi)RNAs by Dicer and mi/siRNA guide strand selection are important steps in forming the RNA-induced silencing complex (RISC). The role of Dicer binding partner TRBP in these processes remains poorly understood. Here, we solved the solution structure of the two N-terminal dsRNA binding domains (dsRBDs) of TRBP in complex with a functionally asymmetric siRNA using NMR, EPR, and single-molecule spectroscopy. We find that siRNA recognition by the dsRBDs is not sequ ...[more]