Perfusion double-channel micropipette probes for oxygen flux mapping with single-cell resolution.

ABSTRACT: Measuring cellular respiration with single-cell spatial resolution is a significant challenge, even with modern tools and techniques. Here, a double-channel micropipette is proposed and investigated as a probe to achieve this goal by sampling fluid near the point of interest. A finite element model (FEM) of this perfusion probe is validated by comparing simulation results with experimental results of hydrodynamically confined fluorescent molecule diffusion. The FEM is then used to investigate the dependence of the oxygen concentration variation and the measurement signal on system parameters, including the pipette's shape, perfusion velocity, position of the oxygen sensors within the pipette, and proximity of the pipette to the substrate. The work demonstrates that the use of perfusion dou

SUBMITTER: Gao Y 

PROVIDER: S-EPMC5852649 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature