Monitoring T cell-dendritic cell interactions in vivo by intercellular enzymatic labelling.

ABSTRACT: Interactions between different cell types are essential for multiple biological processes, including immunity, embryonic development and neuronal signalling. Although the dynamics of cell-cell interactions can be monitored in vivo by intravital microscopy, this approach does not provide any information on the receptors and ligands involved or enable the isolation of interacting cells for downstream analysis. Here we describe a complementary approach that uses bacterial sortase A-mediated cell labelling across synapses of immune cells to identify receptor-ligand interactions between cells in living mice, by generating a signal that can subsequently be detected ex vivo by flow cytometry. We call this approach for the labelling of 'kiss-and-run' interactions between immune cells 'Labelling Im

SUBMITTER: Pasqual G 

PROVIDER: S-EPMC5853129 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature