Sorting of SEC translocase SCY components to different membranes in chloroplasts.

ABSTRACT: Membrane proteins that are imported into chloroplasts must be accurately routed in order to establish and maintain the highly differentiated membranes characteristic of these organelles. Little is known about the targeting information or pathways involved, especially in the case of proteins with multiple transmembrane domains. We have studied targeting of the SCY components of the two SEC translocases in chloroplasts. SCY1 and SCY2 share a similar, highly conserved structure with 10 transmembrane domains, but are targeted to different membranes: the thylakoids and inner envelope, respectively. We used protoplast transfections and a confocal microscopy imaging assay in combination with a domain-swapping approach to investigate sorting pathways and identify important targeting elements in th

SUBMITTER: Singhal R 

PROVIDER: S-EPMC5853536 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature