Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases.

ABSTRACT: Interactions between metabolites and proteins play an integral role in all cellular functions. Here we describe an affinity purification (AP) approach in combination with LC/MS-based metabolomics and proteomics that allows, to our knowledge for the first time, analysis of protein-metabolite and protein-protein interactions simultaneously in plant systems. More specifically, we examined protein and small-molecule partners of the three (of five) nucleoside diphosphate kinases present in the Arabidopsis genome (NDPK1-NDPK3). The bona fide role of NDPKs is the exchange of terminal phosphate groups between nucleoside diphosphates (NDPs) and triphosphates (NTPs). However, other functions have been reported, which probably depend on both the proteins and small molecules specifically interacting w

SUBMITTER: Luzarowski M 

PROVIDER: S-EPMC5853561 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature