Freeze-quenched maize mesophyll and bundle sheath separation uncovers bias in previous tissue-specific RNA-Seq data.

ABSTRACT: The high efficiency of C₄ photosynthesis relies on spatial division of labor, classically with initial carbon fixation in the mesophyll and carbon reduction in the bundle sheath. By employing grinding and serial filtration over liquid nitrogen, we enriched C₄ tissues along a developing leaf gradient. This method treats both C₄ tissues in an integrity-preserving and consistent manner, while allowing complementary measurements of metabolite abundance and enzyme activity, thus providing a comprehensive data set. Meta-analysis of this and the previous studies highlights the strengths and weaknesses of different C₄ tissue separation techniques. While the method reported here achieves the least enrichment, it is the only one that shows neither strong 3

SUBMITTER: Denton AK 

PROVIDER: S-EPMC5853576 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature