Effects of reduced carbonic anhydrase activity on CO2 assimilation rates in Setaria viridis: a transgenic analysis.

ABSTRACT: In C₄ species, the major β-carbonic anhydrase (β-CA) localized in the mesophyll cytosol catalyses the hydration of CO₂ to HCO₃^-, which phosphoenolpyruvate carboxylase uses in the first step of C₄ photosynthesis. To address the role of CA in C₄ photosynthesis, we generated transgenic Setaria viridis depleted in β-CA. Independent lines were identified with as little as 13% of wild-type CA. No photosynthetic defect was observed in the transformed lines at ambient CO₂ partial pressure (pCO₂). At low pCO₂, a strong correlation between CO₂ assimilation rates and CA hydration rates was observed. C¹⁸O¹⁶O isotope discrimination was used to estimate the mesophyll conduc

SUBMITTER: Osborn HL 

PROVIDER: S-EPMC5853810 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature