The Surface Protein SdrF Mediates Staphylococcus epidermidis Adherence to Keratin.

ABSTRACT:

Background

Staphylococcus epidermidis, a major component of skin flora, is an opportunist, often causing prosthetic device infections. A family of structurally related proteins mediates staphylococcal attachment to host tissues, contributing to the success of S. epidermidis as a pathogen. We examined the ability of the surface protein SdrF to adhere to keratin, a major molecule expressed on the skin surface.

Methods

A heterologous Lactococcus lactis expression system was used to express SdrF and its ligand-binding domains. Adherence to keratin types 1 and 10, human foreskin keratinocytes, and nasal epithelial cells was examined.

Results

SdrF bound human keratins 1 and 10 and adhered to keratinocytes and epithelial cells. Binding involved both the A and B domains. Anti

SUBMITTER: Trivedi S 

PROVIDER: S-EPMC5853823 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature