An ultrasensitive fluorogenic probe for revealing the role of glutathione in chemotherapy resistance.

ABSTRACT: Unveiling the detailed roles of glutathione (GSH) in chemoresistance necessitates a reliable assay for its detection in intact live specimens. Herein, by taking advantage of the susceptibility of electron-poor C_sp² -S_sufinyl bond to GSH nucleophilic attack, we developed a naphthalimide-sulfoxide based fluorogenic probe (Na-8) applicable for tracking endogenous GSH fluctuation in live cells. Na-8 features a high degree of sensitivity towards GSH as demonstrated by its utmost 2200-fold fluorogenic response. As a proof of concept, Na-8 has been applied to image GSH in liver cancer HepG2 cells with the normal L02 cell counterparts serving as a control, and elevated GSH level was observed in HepG2 in contrast to L02. Further experiments show

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC5853925 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature