Replication of Zika Virus in Human Prostate Cells: A Potential Source of Sexually Transmitted Virus.

ABSTRACT:

Background

While Zika virus (ZIKV) is mainly transmitted by mosquitoes, numerous cases of sexual transmission have been reported during recent outbreaks. Little is known about which host cell types or entry factors aid in mediating this sexual transmission.

Methods

In this study, we investigated ZIKV cell tropism by infecting 2 types of human prostate cells with 3 contemporary ZIKV isolates from persons infected in the Americas. We used real-time quantitative polymerase chain reaction and immunofluorescence analyses to measure infection and flow cytometry to detect entry factor expression.

Results

Here we show that ZIKV infects, replicates, and produces infectious virus in prostate stromal mesenchymal stem cells, epithelial cells, and organoids made with a combination

SUBMITTER: Spencer JL 

PROVIDER: S-EPMC5853941 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature