Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases.

ABSTRACT: The unique translocation and DNA unwinding properties of DNA helicases can be concealed by the stochastic behavior of enzyme molecules within the necessarily large populations used in ensemble experiments. With recent technological advances, the direct visualization of helicases acting on individual DNA molecules has contributed significantly to the current understanding of their mechanisms of action and biological functions. The combination of single-molecule techniques that enable both manipulation of individual protein or DNA molecules and visualization of their actions has made it possible to literally see novel and unique biochemical characteristics that were previously masked. Here, we describe the execution and use of single-molecule fluorescence imaging techniques, focusing on meth

SUBMITTER: Pavankumar TL 

PROVIDER: S-EPMC5854184 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature