Myc Regulates Chromatin Decompaction and Nuclear Architecture during B Cell Activation.

ABSTRACT: 50 years ago, Vincent Allfrey and colleagues discovered that lymphocyte activation triggers massive acetylation of chromatin. However, the molecular mechanisms driving epigenetic accessibility are still unknown. We here show that stimulated lymphocytes decondense chromatin by three differentially regulated steps. First, chromatin is repositioned away from the nuclear periphery in response to global acetylation. Second, histone nanodomain clusters decompact into mononucleosome fibers through a mechanism that requires Myc and continual energy input. Single-molecule imaging shows that this step lowers transcription factor residence time and non-specific collisions during sampling for DNA targets. Third, chromatin interactions shift from long range to predominantly short range, and CTCF-mediat

SUBMITTER: Kieffer-Kwon KR 

PROVIDER: S-EPMC5854204 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature