Identifying optimal reference genes for the normalization of microRNA expression in cucumber under viral stress.

ABSTRACT: Cucumber green mottle mosaic virus (CGMMV) is an economically important pathogen and causes significant reduction of both yield and quality of cucumber (Cucumis sativus). Currently, there were no satisfied strategies for controlling the disease. A better understanding of microRNA (miRNA) expression related to the regulation of plant-virus interactions and virus resistance would be of great assistance when developing control strategies for CGMMV. However, accurate expression analysis is highly dependent on robust and reliable reference gene used as an internal control for normalization of miRNA expression. Most commonly used reference genes involved in CGMMV-infected cucumber are not universally expressed depending on tissue types and stages of plant development. It is therefore crucial to

SUBMITTER: Liang C 

PROVIDER: S-EPMC5854380 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature