Synchronous termination of replication of the two chromosomes is an evolutionary selected feature in Vibrionaceae.

ABSTRACT: Vibrio cholerae, the causative agent of the cholera disease, is commonly used as a model organism for the study of bacteria with multipartite genomes. Its two chromosomes of different sizes initiate their DNA replication at distinct time points in the cell cycle and terminate in synchrony. In this study, the time-delayed start of Chr2 was verified in a synchronized cell population. This replication pattern suggests two possible regulation mechanisms for other Vibrio species with different sized secondary chromosomes: Either all Chr2 start DNA replication with a fixed delay after Chr1 initiation, or the timepoint at which Chr2 initiates varies such that termination of chromosomal replication occurs in synchrony. We investigated these two models and revealed that the two chromosomes of vario

SUBMITTER: Kemter FS 

PROVIDER: S-EPMC5854411 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature