TnSeq of Mycobacterium tuberculosis clinical isolates reveals strain-specific antibiotic liabilities.

ABSTRACT: Once considered a phenotypically monomorphic bacterium, there is a growing body of work demonstrating heterogeneity among Mycobacterium tuberculosis (Mtb) strains in clinically relevant characteristics, including virulence and response to antibiotics. However, the genetic and molecular basis for most phenotypic differences among Mtb strains remains unknown. To investigate the basis of strain variation in Mtb, we performed genome-wide transposon mutagenesis coupled with next-generation sequencing (TnSeq) for a panel of Mtb clinical isolates and the reference strain H37Rv to compare genetic requirements for in vitro growth across these strains. We developed an analytic approach to identify quantitative differences in genetic requirements between these genetically diverse strains, which vary

SUBMITTER: Carey AF 

PROVIDER: S-EPMC5854444 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature