Zebrafish Rfx4 controls dorsal and ventral midline formation in the neural tube.

ABSTRACT: BACKGROUND:Rfx winged-helix transcription factors, best known as key regulators of core ciliogenesis, also play ciliogenesis-independent roles during neural development. Mammalian Rfx4 controls neural tube morphogenesis via both mechanisms. RESULTS:We set out to identify conserved aspects of rfx4 gene function during vertebrate development and to establish a new genetic model in which to analyze these mechanisms further. To this end, we have generated frame-shift alleles in the zebrafish rfx4 locus using CRISPR/Cas9 mutagenesis. Using RNAseq-based transcriptome analysis, in situ hybridization and immunostaining we identified a requirement for zebrafish rfx4 in the forming midlines of the caudal neural tube. These functions are mediated, least in part, through transcriptional regulation of

SUBMITTER: Sedykh I 

PROVIDER: S-EPMC5854527 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature