Varied Length Stokes Shift BODIPY-Based Fluorophores for Multicolor Microscopy.

ABSTRACT: Multicolor microscopy tools necessary to localize and visualize the complexity of subcellular systems are limited by current fluorophore technology. While commercial fluorophores cover spectral space from the ultraviolet to the near infrared region and are optimized for conventional bandpass based fluorescence microscopy, they are not ideal for highly multiplexed fluorescence microscopy as they tend to have short Stokes shifts, restricting the number of fluorophores that can be detected in a single sample to four to five. Herein, we synthesized a library of 95 novel boron-dipyrromethene (BODIPY)-based fluorophores and screened their photophysical, optical and spectral properties for their utility in multicolor microscopy. A subset of our BODIPY-based fluorophores yielded varied length Stok

SUBMITTER: Bittel AM 

PROVIDER: S-EPMC5854673 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature