Systematic evaluation of the adaptability of the non-radioactive SUnSET assay to measure cardiac protein synthesis.

ABSTRACT: Heart is a dynamic organ that undergoes remodeling in response to both physiological and pathological stimuli. One of the fundamental cellular processes that facilitates changes in the size and shape of this muscular organ is the protein synthesis. Traditionally changes in cardiac protein synthesis levels were measured by radiolabeled tracers. However, these methods are often cumbersome and suffer from radioactive risk. Recently a nonradioactive method for detecting protein synthesis under in vitro conditions called the Surface Sensing of Translation (SUnSET) was described in cell lines of mouse dendrites and T cells. In this work, we provide multiple lines of evidence that the SUnSET assay can be applied to reliably detect changes in protein synthesis both in isolated neonatal primary car

SUBMITTER: Ravi V 

PROVIDER: S-EPMC5854694 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature