Grass Carp Prolactin Gene: Structural Characterization and Signal Transduction for PACAP-induced Prolactin Promoter Activity.

ABSTRACT: In this study, structural analysis of grass carp prolactin (PRL) gene was performed and the signaling mechanisms for pituitary adenylate cyclase-activating peptide (PACAP) regulation of PRL promoter activity were investigated. In αT3-1 cells, PRL promoter activity could be induced by oPACAP₃₈ which was blocked by PACAP antagonist but not the VIP antagonist. The stimulatory effect of oPACAP₃₈ was mimicked by activation of AC/cAMP and voltage-sensitive Ca²⁺ channel (VSCC) signaling, or induction of Ca²⁺ entry. In parallel, PACAP-induced PRL promoter activity was negated or inhibited by suppressing cAMP production, inhibiting PKA activity, removal of extracellular Ca²⁺, VSCC blockade, calmodulin (CaM) antagonism, and inactivation of CaM k

SUBMITTER: Lin C 

PROVIDER: S-EPMC5854708 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature