Mapping causal mutations by exome sequencing in a wheat TILLING population: a tall mutant case study.

ABSTRACT: Forward genetic screens of induced mutant plant populations are powerful tools to identify genes underlying phenotypes of interest. Using traditional techniques, mapping causative mutations from forward screens is a lengthy, multi-step process, requiring the identification of a broad genetic region followed by candidate gene sequencing to characterize the causal variant. Mapping by whole genome sequencing accelerates the identification of causal mutations by simultaneously defining a mapping region and providing information on the induced genetic variants. In wheat, although the availability of a high-quality draft genome assembly facilitates mapping and mutation calling, whole genome resequencing remains prohibitively expensive due to its large genome. In the current study, we used exome

SUBMITTER: Mo Y 

PROVIDER: S-EPMC5854723 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature