Astroglial DJ-1 over-expression up-regulates proteins involved in redox regulation and is neuroprotective in vivo.

ABSTRACT: DJ-1, a Parkinson's disease-associated protein, is strongly up-regulated in reactive astrocytes in Parkinson's disease. This is proposed to represent a neuronal protective response, although the mechanism has not yet been identified. We have generated a transgenic zebrafish line with increased astroglial DJ-1 expression driven by regulatory elements from the zebrafish GFAP gene. Larvae from this transgenic line are protected from oxidative stress-induced injuries as caused by MPP⁺, a mitochondrial complex I inhibitor shown to induce dopaminergic cells death. In a global label-free proteomics analysis of wild type and transgenic larvae exposed to MPP⁺, 3418 proteins were identified, in which 366 proteins were differentially regulated. In particular, we identified enzym

SUBMITTER: Froyset AK 

PROVIDER: S-EPMC5854894 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature