Human Papillomavirus Type 16 Integration Analysis by Real-time PCR Assay in Associated Cancers.

ABSTRACT: Human papillomavirus (HPV) is a common viral infection worldwide associated with a variety of cancers. The integration of the HPV genome in these patients causes chromosomal instability and triggers carcinogenesis. The aim of this study was to investigate the HPV-16 genome physical status in four major cancers related to HPV infection. Formalin-fixed paraffin-embedded blocks from our previous projects on head and neck, colorectal, penile, and cervical cancers were collected, and HPV-16-positive specimens were used for further analysis. The DNA extraction copy number of E2 and E7 genes was calculated by qualitative real-time PCR method. Serially diluted standards that were cloned in PUC57 plasmid were used. Standard curve and melting curve analysis was used for quantification. Of the 672 sp

SUBMITTER: Karbalaie Niya MH 

PROVIDER: S-EPMC5854915 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature