Visual Estimation of Bacterial Growth Level in Microfluidic Culture Systems.

ABSTRACT: Microfluidic devices are an emerging platform for a variety of experiments involving bacterial cell culture, and has advantages including cost and convenience. One inevitable step during bacterial cell culture is the measurement of cell concentration in the channel. The optical density measurement technique is generally used for bacterial growth estimation, but it is not applicable to microfluidic devices due to the small sample volumes in microfluidics. Alternately, cell counting or colony-forming unit methods may be applied, but these do not work in situ; nor do these methods show measurement results immediately. To this end, we present a new vision-based method to estimate the growth level of the bacteria in microfluidic channels. We use Fast Fourier transform (FFT) to detect the freque

SUBMITTER: Kim K 

PROVIDER: S-EPMC5855051 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature