High-Throughput Sequencing Strategy for Microsatellite Genotyping Using Neotropical Fish as a Model.

ABSTRACT: Genetic diversity and population studies are essential for conservation and wildlife management programs. However, monitoring requires the analysis of multiple loci from many samples. These processes can be laborious and expensive. The choice of microsatellites and PCR calibration for genotyping are particularly daunting. Here we optimized a low-cost genotyping method using multiple microsatellite loci for simultaneous genotyping of up to 384 samples using next-generation sequencing (NGS). We designed primers with adapters to the combinatorial barcoding amplicon library and sequenced samples by MiSeq. Next, we adapted a bioinformatics pipeline for genotyping microsatellites based on read-length and sequence content. Using primer pairs for eight microsatellite loci from

SUBMITTER: Pimentel JSM 

PROVIDER: S-EPMC5855144 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature