Activation of the Notch Signaling Pathway In Vivo Elicits Changes in CSL Nuclear Dynamics.

ABSTRACT: A key feature of Notch signaling is that it directs immediate changes in transcription via the DNA-binding factor CSL, switching it from repression to activation. How Notch generates both a sensitive and accurate response-in the absence of any amplification step-remains to be elucidated. To address this question, we developed real-time analysis of CSL dynamics including single-molecule tracking in vivo. In Notch-OFF nuclei, a small proportion of CSL molecules transiently binds DNA, while in Notch-ON conditions CSL recruitment increases dramatically at target loci, where complexes have longer dwell times conferred by the Notch co-activator Mastermind. Surprisingly, recruitment of CSL-related corepressors also increases in Notch-ON conditions, revealing that Notch induces cooperative or "ass

SUBMITTER: Gomez-Lamarca MJ 

PROVIDER: S-EPMC5855320 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature