Discovery of Cryoprotective Activity in Human Genome-Derived Intrinsically Disordered Proteins.

ABSTRACT: Intrinsically disordered proteins (IDPs) are an emerging phenomenon. They may have a high degree of flexibility in their polypeptide chains, which lack a stable 3D structure. Although several biological functions of IDPs have been proposed, their general function is not known. The only finding related to their function is the genetically conserved YSK₂ motif present in plant dehydrins. These proteins were shown to be IDPs with the YSK₂ motif serving as a core region for the dehydrins' cryoprotective activity. Here we examined the cryoprotective activity of randomly selected IDPs toward the model enzyme lactate dehydrogenase (LDH). All five IDPs that were examined were in the range of 35-45 amino acid residues in length and were equally potent at a concentration of 50 μg/mL, whereas folded

SUBMITTER: Matsuo N 

PROVIDER: S-EPMC5855623 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature