HDAC Inhibition Improves the Sarcoendoplasmic Reticulum Ca²⁺-ATPase Activity in Cardiac Myocytes.

ABSTRACT: SERCA2a is the Ca²⁺ ATPase playing the major contribution in cardiomyocyte (CM) calcium removal. Its activity can be regulated by both modulatory proteins and several post-translational modifications. The aim of the present work was to investigate whether the function of SERCA2 can be modulated by treating CMs with the histone deacetylase (HDAC) inhibitor suberanilohydroxamic acid (SAHA). The incubation with SAHA (2.5 µM, 90 min) of CMs isolated from rat adult hearts resulted in an increase of SERCA2 acetylation level and improved ATPase activity. This was associated with a significant improvement of calcium transient recovery time and cell contractility. Previous reports have identified K464 as an acetylation site in human SERCA2. Mutants were generated where K464 was substitut

SUBMITTER: Meraviglia V 

PROVIDER: S-EPMC5855641 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature