Dataset Information

Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital.

ABSTRACT: Background:We established a glioma biobank at Beijing Tiantan Hospital in November, 2010. Specialized residents have been trained to collect, store and manage the biobank in accordance with standard operating procedures. Methods:One hundred samples were selected to evaluate the quality of glioma samples stored in the liquid nitrogen tank during different periods (from 2011 to 2015) by morphological examination, RNA integrity determination, DNA integrity determination and housekeeping gene expression determination. Results:The majority of samples (95%) had high RNA quality for further analysis with RIN ?6. Quality of DNA of all samples were stable without significant degradation. Conclusion:Storage conditions of our biobank are suitable for long-term (at least five years) sample preservation with high molecular quality.

SUBMITTER: Kong F

PROVIDER: S-EPMC5855883 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital.

Kong Fanhong F Zhang Wenli W Qiao Lin L Li Qi Q Li Haowen H Cao Jingli J He Wenyan W Dong Chengya C He Yanjiao Y He Lu L Liu Li L Fu Weilun W Liu Lijun L Li Zirui Z Wang Yajie Y

PeerJ 20180313

<h4>Background</h4>We established a glioma biobank at Beijing Tiantan Hospital in November, 2010. Specialized residents have been trained to collect, store and manage the biobank in accordance with standard operating procedures.<h4>Methods</h4>One hundred samples were selected to evaluate the quality of glioma samples stored in the liquid nitrogen tank during different periods (from 2011 to 2015) by morphological examination, RNA integrity determination, DNA integrity determination and housekeep ...[more]

PMID: 29576945

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Project description:BackgroundBiobanks are highly organized infrastructures that allow the storage of human biological specimens associated with donors' personal and clinical data. These infrastructures play a key role in the development of translational medical research. In this context, we launched, in November 2015, the first biobank in Morocco (BRO Biobank) in order to promote biomedical research and provide opportunities to include Moroccan and North African ethnic groups in international biomedical studies. Here, we present the setup and the sample characteristics of BRO Biobank.MethodsPatients were recruited at several departments of two major health-care centers in the city of Oujda. Healthy donors were enrolled during blood donation campaigns all over Eastern Morocco. From each participant, personal, clinical, and biomedical data were collected, and several biospecimens were stored. Standard operating procedures have been established in accordance with international guidelines on human biobanks.ResultsBetween November 2015 and July 2020, 2446 participants were recruited into the BRO Biobank, of whom 2013 were healthy donors, and 433 were patients. For healthy donors, the median age was 35 years with a range between 18 and 65 years and the consanguinity rate was 28.96%. For patients, the median age was 11 years with a range between 1 day and 83 years. Among these patients, 55% had rare diseases (hemoglobinopathies, intellectual disabilities, disorders of sex differentiation, myopathies, etc.), 13% had lung cancer, 4% suffered from hematological neoplasms, 3% were from the kidney transplantation project, and 25% had unknown diagnoses. The BRO Biobank has collected 5092 biospecimens, including blood, white blood cells, plasma, serum, urine, frozen tissue, FFPE tissue, and nucleic acids. A sample quality control has been implemented and suggested that samples of the BRO Biobank are of high quality and therefore suitable for high-throughput nucleic acid analysis.ConclusionsThe BRO Biobank is the largest sample collection in Morocco, and it is ready to provide samples to national and international research projects. Therefore, the BRO Biobank is a valuable resource for advancing translational medical research.

Dataset Information

Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital.

Publications

Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital.

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