Identification of miR-29b targets using 3-cyanovinylcarbazole containing mimics.

ABSTRACT: MicroRNAs (miRNAs) are highly conserved ∼22 nt small noncoding RNAs that bind partially complementary sequences in target transcripts. MicroRNAs regulate both translation and transcript stability, and play important roles in development, cellular homeostasis, and disease. There are limited approaches available to agnostically identify microRNA targets transcriptome-wide, and methods using miRNA mimics, which in principle identify direct miRNA:transcript pairs, have low sensitivity and specificity. Here, we describe a novel method to identify microRNA targets using miR-29b mimics containing 3-cyanovinylcarbazole (^CNVK), a photolabile nucleoside analog. We demonstrate that biotin-tagged, ^CNVK-containing miR-29b (^CNVK-miR-29b) mimics are nontoxic in cell cultu

SUBMITTER: Choudhary A 

PROVIDER: S-EPMC5855958 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature