Assaying sensory ciliopathies using calcium biosensor expression in zebrafish ciliated olfactory neurons.

ABSTRACT:

Background

Primary cilia mediate signal transduction by acting as an organizing scaffold for receptors, signalling proteins and ion channels. Ciliated olfactory sensory neurons (OSNs) organize olfactory receptors and ion channels on cilia and generate a calcium influx as a primary signal in odourant detection. In the zebrafish olfactory placode, ciliated OSNs and microvillus OSNs constitute the major OSN cell types with distinct odourant sensitivity.

Methods

Using transgenic expression of the calcium biosensor GCaMP5 in OSNs, we analysed sensory cilia-dependent odour responses in live zebrafish, at individual cell resolution. oval/ift88 mutant and ift172 knockdown zebrafish were compared with wild-type siblings to establish ciliated OSN sensitivity to different

SUBMITTER: Bergboer JGM 

PROVIDER: S-EPMC5856005 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature