Renal Tubular Cell Mitochondrial Dysfunction Occurs Despite Preserved Renal Oxygen Delivery in Experimental Septic Acute Kidney Injury.

ABSTRACT:

Objective

To explain the paradigm of significant renal functional impairment despite preserved hemodynamics and histology in sepsis-induced acute kidney injury.

Design

Prospective observational animal study.

Setting

University research laboratory.

Subjects

Male Wistar rats.

Intervention

Using a fluid-resuscitated sublethal rat model of fecal peritonitis, changes in renal function were characterized in relation to global and renal hemodynamics, and histology at 6 and 24 hours (n = 6-10). Sham-operated animals were used as comparison (n = 8). Tubular cell mitochondrial function was assessed using multiphoton confocal imaging of live kidney slices incubated in septic serum.

Measurements and main results

By 24 hours, serum creatinine was significantly

SUBMITTER: Arulkumaran N 

PROVIDER: S-EPMC5856355 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature