Multi-color fluorescent reporter dengue viruses with improved stability for analysis of a multi-virus infection.

ABSTRACT: Reporter virus is a versatile tool to visualize and to analyze virus infections. However, for flaviviruses, it is difficult to maintain the inserted reporter genes on the viral genome, limiting its use in several studies that require homogeneous virus particles and several rounds of virus replication. Here, we showed that flanking inserted GFP genes on both sides with ribosome-skipping 2A sequences improved the stability and the consistency of their fluorescent signals for dengue-virus-serotype 2 (DENV2) reporter viruses. The reporter viruses can infect known susceptible mammalian cell lines and primary CD14+ human monocytes. This design can accommodate several fluorescent protein genes, enabling the generation of multi-color DENV2-16681 reporter viruses with comparable replication capabil

SUBMITTER: Suphatrakul A 

PROVIDER: S-EPMC5856417 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature