Highly multiplexed and quantitative cell-surface protein profiling using genetically barcoded antibodies.

ABSTRACT: Human cells express thousands of different surface proteins that can be used for cell classification, or to distinguish healthy and disease conditions. A method capable of profiling a substantial fraction of the surface proteome simultaneously and inexpensively would enable more accurate and complete classification of cell states. We present a highly multiplexed and quantitative surface proteomic method using genetically barcoded antibodies called phage-antibody next-generation sequencing (PhaNGS). Using 144 preselected antibodies displayed on filamentous phage (Fab-phage) against 44 receptor targets, we assess changes in B cell surface proteins after the development of drug resistance in a patient with acute lymphoblastic leukemia (ALL) and in adaptation to oncogene expression in a Myc-in

SUBMITTER: Pollock SB 

PROVIDER: S-EPMC5856557 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature