Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis.

ABSTRACT: Mesenchymal stem cells (MSCs) have been widely studied with regard to their potential use in cell therapy protocols and regenerative medicine. However, a better comprehension about the factors and molecular mechanisms driving cell differentiation is now mandatory to improve our chance to manipulate MSC behavior and to benefit future applications. In this work, we aimed to study gene regulatory networks at an early step of osteogenic differentiation. Therefore, we analyzed both the total mRNA and the mRNA fraction associated with polysomes on human adipose tissue-derived stem cells (hASCs) at 24 h of osteogenesis induction. The RNA-seq results evidenced that hASC fate is not compromised with osteogenesis at this time and that 21 days of continuous cell culture stimuli are necessary for full

SUBMITTER: Robert AW 

PROVIDER: S-EPMC5856793 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature