A novel synthetic peptide microarray assay detects Chlamydia species-specific antibodies in animal and human sera.

ABSTRACT: Serological analysis of Chlamydia (C.) spp. infections is still mainly based on micro-immunofluorescence and ELISA. To overcome the limitations of conventional serology, we have designed a novel microarray carrying 52 synthetic peptides representing B-cell epitopes from immunodominant proteins of all 11 chlamydial species. The new assay has been validated using monospecific mouse hyperimmune sera. Subsequently, serum samples from cattle, sheep and humans with a known history of chlamydial infection were examined. For instance, the specific humoral response of sheep to treatment with a C. abortus vaccine has been visualized against a background of C. pecorum carriership. In samples from humans, dual infection with C. trachomatis and C. pneumoniae could be demonstrated. The experiments revea

SUBMITTER: Sachse K 

PROVIDER: S-EPMC5856796 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature