Association of RAGE gene polymorphism with Type-2 diabetes mellitus in local population.

ABSTRACT:

Objectives

Type-2 diabetes mellitus (T2DM) is an endocrine disease having a significant genetic component. Polymorphisms of many genes may affect hereditary vulnerability of the disease that is characterized by insulin resistance and islet disorder. As the genetic basis of T2DM can vary between ethnic groups, it is important to investigate the genetic link of T2DM in Pakistani populace. This study was aimed to assess the association of receptor for advanced glycation end product (RAGE) gene polymorphism (-429T>C) with Type-2 diabetes mellitus within local populace.

Methods

Genomic DNA was isolated by following kit protocol. Genotyping of the RAGE gene was studied by PCR-RFLP on genomic DNA. All research work was done in molecular biochemistry laboratory (MBL), University of

SUBMITTER: Zulfiqar S 

PROVIDER: S-EPMC5857018 | biostudies-literature | 2018 Jan-Feb

REPOSITORIES: biostudies-literature