Influence of cell type and cell culture media on the propagation of foot-and-mouth disease virus with regard to vaccine quality.

ABSTRACT:

Background

Suspension culture of BHK cells allows large-scale virus propagation and cost-efficient vaccine production, while the shift to animal-component-free cell culture media without serum is beneficial for the quality and downstream processing of the product. Foot-and-mouth disease virus is still endemic in many parts of the world and high-quality vaccines are essential for the eradication of this highly contagious and economically devastating disease.

Methods

Changes to the viral genome sequence during passaging in an adherent and a suspension cell culture system were compared and the impact of amino acid substitutions on receptor tropism, antigenicity and particle stability was examined. Virus production in suspension cells in animal-component-free media and in serum-

SUBMITTER: Dill V 

PROVIDER: S-EPMC5857075 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature