Biochemical alterations in inflammatory reactive chondrocytes: evidence for intercellular network communication.

ABSTRACT: Chondrocytes are effectively involved in the pathophysiological processes of inflammation in joints. They form cellular processes in the superficial layer of the articular cartilage and form gap junction coupled syncytium to facilitate cell-to-cell communication. However, very little is known about their physiological cellular identity and communication. The aim with the present work is to evaluate the physiological behavior after stimulation with the inflammatory inducers interleukin-1β and lipopolysaccharide. The cytoskeleton integrity and intracellular Ca2+ release were assessed as indicators of inflammatory state. Cytoskeleton integrity was analyzed through cartilage oligomeric matrix protein and actin labeling with an Alexa 488-conjugated phalloidin probe. Ca2+ responses were assessed

SUBMITTER: Skioldebrand E 

PROVIDER: S-EPMC5857518 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature