Antibody secreting B cells and plasma antibody response to rotavirus vaccination in infants from Kolkata India.

ABSTRACT:

Background

Assessing immune response after rotavirus vaccination consists in measuring serum or plasma IgA and IgG antibodies, but these assays provide very little information about the mucosal immune response. Thus the development of assays for detection of mucosal immune response following rotavirus vaccination is essential. We evaluate to assess circulating antibody-secreting cells (ASCs) as a potential means to evaluate mucosal immune responses to rotavirus vaccine.

Methods

372 subjects, aged 6 weeks, were enrolled in the study. All the subjects were assigned to receive two doses of Rotarix^® vaccine. Using a micro-modified whole blood-based ELISPOT assay, circulating rotavirus type-specific IgA- and IgG-ASCs, including gut homing β7+ ASCs, were enumerated on w

SUBMITTER: Sinha A 

PROVIDER: S-EPMC5857522 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature