Development of single step RT-PCR for detection of Kyasanur forest disease virus from clinical samples.

ABSTRACT:

Background

Kyasanur Forest Disease (KFD), a tick borne flavivirus, which was earlier endemic to Karnataka state, India, has been confirmed and detected from neighboring states of Tamil Nadu, Maharashtra, Goa and Kerala states in India. Increased human and vector surveillance therefore becomes essential for the identification of KFD affected regions and control of further spread of the disease. Currently, available KFD detection assays include realtime RT-PCR and nested RT-PCR assays. Here we describe the development of a sensitive single step RT-PCR assay for the detection of KFD viral RNA. This can be easily used in any BSL-2 laboratory for screening of KFD suspected cases or for differential diagnosis of viral hemorrhagic fever panel.

Method

Three primer sets were designed

SUBMITTER: Chaubal G 

PROVIDER: S-EPMC5857640 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature