Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood.

ABSTRACT: Recently, many studies have demonstrated the significant advantages of loop-mediated isothermal amplification (LAMP) based methods over serological tests and PCR for rapid detection of microbial pathogens. Here, a rapid LAMP assay was developed to detect the hepatitis B virus (HBV) from DNA, and particularly, blood samples from infected patients using a commercially available master mix and portable real-time fluorometer. The final optimized fluorescence-based LAMP assay provided significant amplification time of less than 15 minutes compared with over 1 hour for PCR and an opened tube LAMP system described previously. Results indicated that fluorescence-based LAMP assay was more sensitive than PCR as a rapid, sensitive, efficient, and highly reliable approach for rapid detection of HBV.

SUBMITTER: Quoc NB 

PROVIDER: S-EPMC5857714 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature