Image processing for cryogenic transmission electron microscopy of symmetry-mismatched complexes.

ABSTRACT: Cryogenic transmission electron microscopy (cryo-TEM) is a high-resolution biological imaging method, whereby biological samples, such as purified proteins, macromolecular complexes, viral particles, organelles and cells, are embedded in vitreous ice preserving their native structures. Due to sensitivity of biological materials to the electron beam of the microscope, only relatively low electron doses can be applied during imaging. As a result, the signal arising from the structure of interest is overpowered by noise in the images. To increase the signal-to-noise ratio, different image processing-based strategies that aim at coherent averaging of signal have been devised. In such strategies, images are generally assumed to arise from multiple identical copies of the structure. Prior to ave

SUBMITTER: Huiskonen JT 

PROVIDER: S-EPMC5857907 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature