Salidroside Inhibits Myogenesis by Modulating p-Smad3-Induced Myf5 Transcription.

ABSTRACT: Aim: Salidroside is an active compound extracted from Rhodiola rosea which is used to alleviate fatigue and enhance endurance in high altitude regions. Some studies have demonstrated that salidroside can affect precursor cell differentiation in hematopoietic stem cells, erythrocytes, and osteoblasts. The aim of this study was to investigate the effect of salidroside on myoblast differentiation and to explore the underlying molecular mechanisms of this effect. Methods: C2C12 myoblast cells were treated with different concentrations of salidroside in differentiation media. Real-time PCR, Western blotting, and immunofluorescence assay were employed to evaluate the effects of salidroside on C2C12 differentiation. RNA interference was used to reveal the important role of My

SUBMITTER: Zhang P 

PROVIDER: S-EPMC5858519 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature