Genomic and Proteomic Resolution of Heterochromatin and Its Restriction of Alternate Fate Genes.

ABSTRACT: Heterochromatin is integral to cell identity maintenance by impeding the activation of genes for alternate cell fates. Heterochromatic regions are associated with histone 3 lysine 9 trimethylation (H3K9me3) or H3K27me3, but these modifications are also found in euchromatic regions that permit transcription. We discovered that resistance to sonication is a reliable indicator of the heterochromatin state, and we developed a biophysical method (gradient-seq) to discriminate subtypes of H3K9me3 and H3K27me3 domains in sonication-resistant heterochromatin (srHC) versus euchromatin. These classifications are more accurate than the histone marks alone in predicting transcriptional silence and resistance of alternate fate genes to activation during direct cell conversion. Our proteomics of H3K9me3

SUBMITTER: Becker JS 

PROVIDER: S-EPMC5858919 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature