Quantifying autophagy using novel LC3B and p62 TR-FRET assays.
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ABSTRACT:
SUBMITTER: Bresciani A
PROVIDER: S-EPMC5858923 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Publications
Quantifying autophagy using novel LC3B and p62 TR-FRET assays.
Bresciani Alberto A Spiezia Maria Carolina MC Boggio Roberto R Cariulo Cristina C Nordheim Anja A Altobelli Roberta R Kuhlbrodt Kirsten K Dominguez Celia C Munoz-Sanjuan Ignacio I Wityak John J Fodale Valentina V Marchionini Deanna M DM Weiss Andreas A
PloS one 20180319 3
Autophagy is a cellular mechanism that can generate energy for cells or clear misfolded or aggregated proteins, and upregulating this process has been proposed as a therapeutic approach for neurodegenerative diseases. Here we describe a novel set of LC3B-II and p62 time-resolved fluorescence resonance energy transfer (TR-FRET) assays that can detect changes in autophagy in the absence of exogenous labels. Lipidated LC3 is a marker of autophagosomes, while p62 is a substrate of autophagy. These a ...[more]
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