High-throughput BioSorter quantification of relative mitochondrial content and membrane potential in living Caenorhabditis elegans.

ABSTRACT: Mitochondrial respiratory chain disease is caused by a wide range of individually rare genetic disorders that impair cellular energy metabolism. While fluorescence microscopy analysis of nematodes fed MitoTracker Green (MTG) and tetramethylrhodamine ethyl ester (TMRE) can reliably quantify relative mitochondrial density and membrane potential, respectively, in C. elegans models of mitochondrial dysfunction, it is a tedious process with limitations in the number and age of animals that can be studied. A novel, large particle, flow cytometry-based method reported here accelerates and automates the relative quantitation of mitochondrial physiology in nematode populations. Relative fluorescence profiles of nematode populations co-labeled with MTG and TMRE were obtained and analyzed by BioSorte

SUBMITTER: Kwon YJ 

PROVIDER: S-EPMC5858966 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature